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Updated: Sep 11, 2025

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
Published on: April 19, 2013
Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to
1Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, PR China.
Abstract:
Plasma membrane (PM) H+-ATPases, well known as proton pumps, are P-type IIIA ATPases that regulate numerous aspects of plant growth, development and response to environmental stimuli by generating transmembrane pH gradients (ΔpH) and electrochemical potentials (ΔΨ) across the plasma membrane. Nonetheless, the recent determinants of active pH buffering and proton pump-mediated ion homeostasis have not yet been considered and are still a matter of discussion. In this review, we first classify the distinct P-type ATPases and review the mechanism of H+ transport across plasma membranes by PM H+-ATPases, and then focus on how PM H+-ATPases together with cyclic nucleotide-gated channels, cation/proton antiporters and other metabolism-related processes control cytoplasmic pH and ion homeostasis across cell membranes in plants under both normal and stress conditions.
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