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A simple method for evaluating platelet superoxide dismutase.

F Violi, L Iuliano, C Alessandri

    Scandinavian Journal of Clinical and Laboratory Investigation
    |December 1, 1985
    PubMed
    Summary
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    A new spectrophotometric method accurately measures platelet superoxide dismutase (SOD) activity. This simple assay provides reliable SOD levels in healthy individuals, crucial for understanding oxidative stress.

    Area of Science:

    • Biochemistry
    • Hematology
    • Enzymology

    Background:

    • Superoxide dismutase (SOD) is a critical antioxidant enzyme.
    • Platelets play a role in oxidative stress and inflammation.
    • Accurate measurement of platelet SOD is essential for research.

    Purpose of the Study:

    • To develop and validate a simple, rapid spectrophotometric method for quantifying platelet superoxide dismutase (SOD).
    • To establish reference values for platelet SOD in healthy individuals.

    Main Methods:

    • A spectrophotometric assay was developed using platelet lysates.
    • Pyrogallol autoxidation inhibition was measured in a Tris-cacodylic buffer.
    • Contamination by erythrocytes and leukocytes was avoided during platelet preparation.

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  • Interference from hydrogen peroxide, peroxidases, and reducing substances was assessed and excluded.

    • Main Results:

    • The method demonstrated simplicity and rapidity for evaluating platelet SOD.
    • Platelet SOD content in 38 healthy subjects was determined to be 19.1 +/- 4.1 U/10(8) platelets or 35 +/- 7.8 U/mg protein.
    • The assay effectively excluded potential interfering substances.

    Conclusions:

    • The reported spectrophotometric method is reliable for assessing platelet SOD activity.
    • This assay can be valuable for clinical and research applications involving oxidative stress and platelet function.