Complete genome sequence of Lysinibacillus sp. WB86, a potential antimicrobial lanthipeptides producer

Lysinibacillus sp. WB86 was isolated from a cold seep in the South China Sea, and its complete genome was sequenced using Oxford Nanopore Technologies (ONT). The genome consists of a single circular chromosome spanning 4,537,071 bp, with a G + C content of 37 %. FastANI analysis revealed a 99.38 % average nucleotide identity (ANI) with Lysinibacillus sphaericus A1, confirming its classification as a strain of L. sphaericus. Genome annotation identified 4397 protein-coding genes, along with 111 tRNAs and 34 rRNAs. Functional categorization using the COG database assigned 84.6 % of the protein-coding genes to specific roles, with the majority involved in transcription, amino acid transport and metabolism, translation and ribosomal biogenesis, and replication, recombination, and repair. Additionally, antiSMASH-based analysis uncovered eight biosynthetic gene clusters (BGCs), only one of which exhibited high similarity (>50 %) to known BGCs. This cluster was predicted to encode novel antimicrobial lanthipeptides, suggesting potential biotechnological applications.