Sulfonamide Fluorescent Probe Applied in Live Cell Imaging, LFPs, and Electrochemical Sensor

Area of Science:

• Organic Chemistry
• Analytical Chemistry
• Biomedical Imaging

Background:

• Sulfapyridone azo fluorescent probes (SPAFPs) represent a novel class of DMSO-soluble fluorophores.
• Electrophilic substitution reactions provide rapid access to these synthesized molecules.
• The probes exhibit fluorescence with an emission maximum at 766 nm, attributed to the azo bond.

Purpose of the Study:

• To develop and characterize novel SPAFP for live-cell imaging.
• To investigate the application of SPAFP in latent fingerprint analysis.
• To create a sensitive electrochemical sensor for dopamine detection.

Main Methods:

• Synthesis and characterization of SPAFP using HRMS, FT-IR, NMR, UV-vis, and PL-emission spectroscopy.
• Density functional theory (DFT) calculations for electronic structure analysis.
• Fabrication of a SPAFP-modified glassy carbon electrode (SPAFP/MGCE) for electrochemical sensing.

Main Results:

• SPAFPs were successfully synthesized and confirmed, demonstrating fluorescence suitable for imaging.
• Latent fingerprints were visualized under UV light (365 nm), revealing detailed ridge features.
• The SPAFP/MGCE sensor showed enhanced electrocatalytic activity for dopamine detection with a low LOD of 2.5 nM.

Conclusions:

• SPAFPs are versatile fluorescent probes with applications in live-cell imaging and forensic science.
• The developed electrochemical sensor offers a sensitive and efficient method for dopamine quantification.
• The SPAFP-based sensor exhibits diffusion-controlled kinetics, indicating reliable performance.