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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Engineered bacteria launch and control an oncolytic virus.

Zakary S Singer1,2, Jonathan Pabón1, Hsinyen Huang1

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This summary is machine-generated.

Engineered bacteria deliver viral RNA to tumors, initiating oncolytic infections. This CAPPSID platform demonstrates bacterial control over viral replication for enhanced cancer therapy.

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Area of Science:

  • Oncology
  • Microbiology
  • Virology
  • Synthetic Biology

Background:

  • Tumor-targeting bacteria and viruses are explored for cancer therapy.
  • Engineering microbes for therapeutic delivery is an emerging field.

Purpose of the Study:

  • To develop a cooperative microbial platform for oncolytic viral therapy.
  • To engineer bacteria to deliver and control viral replication within tumors.

Main Methods:

  • Salmonella typhimurium bacteria were engineered to transcribe and deliver Senecavirus A RNA.
  • The viral genome was encapsulated within bacteria to evade antibodies.
  • The virus was modified to require a bacterially delivered protease for maturation.

Main Results:

  • Encapsidated viral RNA successfully initiated oncolytic infections in host cells.
  • The bacterial delivery system bypassed antiviral antibodies, reaching tumors in mice.
  • Bacterial protease enabled controlled viral maturation, demonstrating a cooperative system.

Conclusions:

  • The CAPPSID platform enables coordinated microbial action for safe intracellular delivery of viral genomes.
  • This approach extends bacterially delivered therapeutics to viral agents.
  • Consortia of microbes can achieve cooperative therapeutic aims.