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Related Concept Videos

Other Glycolytic Pathways01:24

Other Glycolytic Pathways

215
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
215

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Genetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation.

Graziela C Sedenho1,2, Jéssica C Pacheco1,2, Melanie Gut2

  • 1São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13566-590, Brazil.

Advanced Materials (Deerfield Beach, Fla.)
|August 16, 2025
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Summary
This summary is machine-generated.

Engineered bacteria can now perform selective water oxidation for artificial photosynthesis. This synthetic biology advance reprograms microbial genomes to create efficient, regenerable bioelectrocatalytic platforms.

Keywords:
bilirubin oxidasebiomaterialselectrocatalysiswater oxidation

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Area of Science:

  • Synthetic biology
  • Bioelectrocatalysis
  • Artificial photosynthesis

Background:

  • Programming microbial genomes for catalysis is a synthetic biology frontier.
  • Coordinated control of gene expression, protein localization, folding, and cofactor maturation is crucial for bioelectrocatalysis.
  • Existing challenges hinder the development of efficient microbial catalysts.

Purpose of the Study:

  • To engineer a synthetic operon in Escherichia coli for reprogramming its surfaceome.
  • To achieve selective water oxidation using a microbial platform.
  • To develop a regenerable living material for artificial photosynthesis.

Main Methods:

  • Engineered a synthetic operon in Escherichia coli for surface protein display.
  • Utilized orthogonal isopropyl $eta$-D-1-thiogalactopyranoside (IPTG)-inducible control and codon-optimized expression.
  • Anchored fungal bilirubin oxidase (BOD) to the cell surface using ice nucleation protein.
  • Reconstituted the copper catalytic site post-overexpression to form an active holoenzyme.

Main Results:

  • Successfully displayed functional bilirubin oxidase on the surface of E. coli (BOD-E. coli).
  • The engineered living material achieved water oxidation at a near-zero overpotential (27 mV at pH 9.1).
  • Demonstrated complete suppression of the oxygen reduction reaction.

Conclusions:

  • Microbial platforms can be designed for selective catalysis through genome programming.
  • Engineered living materials offer a regenerable approach for artificial photosynthesis.
  • This work advances bioelectrocatalysis by enabling precise control over microbial catalytic functions.