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A Modular Perfusion Bioreactor Platform for Simulating Bone Regeneration and Fracture Healing: Integrating Mechanical
Moritz Pfeiffenberger1,2, Alexandra Damerau1,2, Johannes Plank1,2
1Department of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, 10117, Berlin, Germany.
Advanced Healthcare Materials
|August 16, 2025
Summary
This study presents a novel bioreactor platform for bone regeneration research, enhancing in vitro models with dynamic perfusion and mechanical stimulation for better physiological replication and insights into mechanobiology.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Tissue Engineering
Background:
- Bone regeneration research requires advanced in vitro models to capture complex physiological dynamics.
- Traditional models lack the ability to replicate bone remodeling and biomechanical properties effectively.
- Bridging the gap between static in vitro models and in vivo conditions is crucial for translational research.
Purpose of the Study:
- To develop and present a modular bioreactor platform for simulating bone homeostasis and disease states.
- To integrate mechanical load simulation with dynamic perfusion for enhanced in vitro bone modeling.
- To investigate mechanobiological insights in bone fracture healing models.
Main Methods:
- A 3D-printed microfluidic chamber with dynamic dual perfusion was developed.
- A mechanical compression device was integrated for precise mechanical stimulation.
- A web interface was utilized for environmental parameter control.
- The platform was applied to an in vitro fracture healing model.
Main Results:
- The bioreactor system prolonged in vitro viability by facilitating the inflammatory-to-anti-inflammatory transition.
- Controlled mechanical stimulation generated functional bone models, yielding mechanobiological insights.
- Dual perfusion enhanced the incubation of composite tissues.
- The system demonstrated improved nutrient delivery, mechanotransduction, and scalability.
Conclusions:
- The developed bioreactor platform advances in vitro tissue modeling by combining perfusion and mechanical stimulation.
- This system offers a more physiologically relevant environment for bone regeneration research.
- The platform shows significant promise for preclinical research, drug testing, and regenerative medicine applications.
