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Updated: Sep 11, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Affect mediates the relationship between physiological and motivational responses to exercise
Izzy G Wellings1, Richard A Ferguson1, Ian M Taylor1
1School of Sport, Exercise & Health Sciences, Loughborough University, Leicestershire, UK.
Physiological responses like blood lactate, heart rate, and body temperature influence exercise motivation and endurance performance. Core affect plays a key role in mediating these relationships during incremental exercise.
Area of Science:
- Exercise Physiology
- Sport Psychology
- Human Performance
Background:
- The interplay between physiological and motivational factors in endurance performance remains unclear.
- Understanding these interactions is crucial for optimizing training and competition strategies.
Purpose of the Study:
- To investigate how changes in blood lactate, heart rate (HR), oxygen uptake (VO2), and body temperature affect the desire to reduce effort and performance goals during incremental exercise.
- To explore the mediating role of core affect in these physiological and motivational relationships.
Main Methods:
- Fifty participants completed an incremental cycling test with increasing work rates.
- Physiological (blood lactate, HR, VO2, body temperature) and psychological (desire to reduce effort, performance goal) measures were collected at each stage.
- Multilevel modeling was used to analyze the within-person relationships.
Main Results:
- Increased blood lactate concentration predicted a greater desire to reduce effort and a lower performance goal.
- Higher oxygen uptake (VO2) and heart rate (HR) were associated with an increased desire to reduce effort.
- Elevated body temperature predicted a higher performance goal.
- Core affect significantly mediated the relationships between blood lactate, VO2, and motivational variables.
Conclusions:
- Physiological responses during exercise have distinct motivational implications for endurance performance.
- Blood lactate, VO2, HR, and body temperature differentially influence desire to reduce effort and performance goals.
- Core affect is an important mediator in the physiological-motivational links during exercise, particularly concerning desire-goal conflicts.
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