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Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo
Published on: July 1, 2013
pH-modulated electrostatic steering enhances SPAAC-mediated peptide grafting on titanium implants
Ru Zhong1, Gaosheng Zhang2, Lin Wang1
1National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China; School of Material Science and Engineering, South China University of Technology, Guangzhou 510006, China; Guangdong Provincial Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, China.
None:
This study investigates the impact of peptide charge states on strain-promoted azide-alkyne cycloaddition (SPAAC)--mediated grafting efficiency on titanium-based substrates. Cationic peptides exhibited superior grafting in a normal aqueous environment due to electrostatic complementarity with negatively charged dibenzocyclooctyne (DBCO)-functionalized surfaces. Anionic peptides required pH modulation to reverse charge disparities, achieving enhanced grafting at lower pH. This pH-tuned strategy enables universal surface functionalization for diverse therapeutic peptides on titanium-based implants.
