A two-photon fluorescent probe for sensitive detection and imaging of hypochlorite in depression model
Mingxiu Wang1, Ling Liu2, Yuqing Qin3
1School of Public Health, Shanxi Medical University, Taiyuan, 030001, PR China; MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, 56 Xinjian South Road, Taiyuan, 030001, PR China; School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing, 100049, PR China.
None:
Hypochlorite (ClO-) is involved in numerous physiological and pathological processes. Real-time monitoring of ClO- levels is essential for understanding its role in diseases. In this study, a novel selenide-based two-photon fluorescent probe Cou-Se was developed for ClO- detection based on coumarin fluorophore and 2-phenylseleno acetate. Cou-Se exhibits a selective and sensitive fluorescence turn-on response to ClO- peaked at 455 nm. The detection limit toward ClO- was determined to be 0.09 μM. Cou-Se was successfully used to image exogenous and endogenous ClO- in living cells. One-photon and two-photon fluorescence imaging of Cou-Se reveals significantly higher levels of ClO- in CORT-induced depressive PC12 cells when compare to normal cells. Brain tissue of depressive mice also exhibited elevated levels of ClO- through two-photon fluorescence imaging. Our work offers a promising tool for advancing diagnostic and therapeutic research on depression.
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