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CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

220
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
220
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

124
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
124
Homologous Recombination02:31

Homologous Recombination

52.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.0K
RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K

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Related Experiment Video

Updated: Sep 11, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

3.2K

CRISPR/Cas system targeting RNA and its derivative technology.

Xun Zhou1,2,3, Shi-Jie Zhou1,2,3, Jie Liu1,2,3

  • 1College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.

Yi Chuan = Hereditas
|August 17, 2025
PubMed
Summary

CRISPR/Cas systems can now precisely edit RNA, offering an epigenetic tool that avoids genomic damage. This technology enables RNA knockdown, editing, detection, and tracking for biological research and disease treatment.

Keywords:
CRISPR/Cas systemRNAfunction and applicationgene editing

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

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Area of Science:

  • Epigenetics and Molecular Biology
  • Gene Editing Technologies

Background:

  • RNA editing is a key area in epigenetics research.
  • CRISPR/Cas systems have been adapted for RNA targeting, distinct from DNA editing.
  • RNA-targeting CRISPR offers a method to modify gene expression without altering the genome.

Purpose of the Study:

  • To review the structure, function, and mechanisms of RNA-targeting CRISPR/Cas systems.
  • To explore the derivative technologies developed from RNA-targeting CRISPR.
  • To enhance understanding of CRISPR/Cas-mediated RNA editing and its applications.

Main Methods:

  • Review of existing literature on RNA-targeting CRISPR/Cas systems.
  • Analysis of the structural and functional components of these systems.
  • Categorization and description of derived technologies (e.g., RNA knockdown, editing, imaging, tracking).

Main Results:

  • CRISPR/Cas systems can be engineered to target RNA, enabling precise modifications.
  • RNA-targeting CRISPR avoids permanent changes to the genome, mitigating off-target risks.
  • Diverse applications have emerged, including RNA knockdown, editing, nucleic acid detection, imaging, and tracking.

Conclusions:

  • RNA-targeting CRISPR/Cas systems represent a significant advancement in epigenetics and molecular biology.
  • These systems provide powerful tools for studying genetic mechanisms and developing novel therapeutic strategies.
  • Further research into CRISPR/Cas-mediated RNA editing will continue to expand its utility in biological sciences and medicine.