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Updated: Sep 11, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
Seeing, Counting, Identifying, and Distinguishing Single Molecules from Their Mixtures via Raman Spectroscopy
Li Hong Hong1,2, Jian Zhi Zhang1, Zi Han Gao1
1School of Physics and Optoelectronics, South China University of Technology, Guangzhou 510640, China.
Abstract:
Seeing, counting, identifying, and distinguishing molecules one by one in their mixture phases is recognized as a big challenge in molecular science. Here we employ this using a single-molecule surface-enhanced Raman spectroscopy (SM-SERS) strategy combining giant electromagnetic enhancement (EME) from plasmonic nanogaps and giant chemical enhancement (CME) from monolayer WS2. For a dilute mixture of rhodamine B (RhB), rhodamine 6G (R6G), and crystal violet (CV) at concentrations of as low as 10-16 M, this SM-SERS substrate enables the sighting, counting, identification, and distinction of individual molecules. High-credibility results are achieved rapidly (50 ms acquisition) using 785 nm near-infrared laser excitation to record and analyze distinct Raman spectra. This EME-CME synergy SM-SERS strategy offers a powerful capability of optically probing and monitoring the spatial-temporal evolution of the physical, chemical, and biological properties of molecules, which would open up a new frontier of molecular science.
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