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Updated: Jun 26, 2026
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
A mitochondria-targeted near-infrared fluorescent probe for detecting H2S, Cys, and GSH simultaneously in tumors
Miaomiao Zhang1,2, Ying Yang1, Zijuan Hai3
1College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
Abstract:
In this study, we developed a mitochondria-targeted near-infrared fluorescent probe HD-Br-D for simultaneous detection of H2S, Cys, and GSH in vitro and in vivo. We anticipate that HD-Br-D will become a powerful tool for diagnosis of diseases, such as tumors, in the near future.
Insights
Researchers created a new fluorescent probe, HD-Br-D, for detecting hydrogen sulfide (H₂S), cysteine (Cys), and glutathione (GSH). This probe works in vitro and in vivo, offering potential for disease diagnosis like tumors.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Imaging
Background:
- Mitochondria play crucial roles in cellular metabolism and disease pathogenesis.
- Accurate detection of reactive sulfur species like hydrogen sulfide (H₂S), cysteine (Cys), and glutathione (GSH) is vital for understanding cellular processes.
- Existing detection methods often lack specificity or simultaneous multi-analyte detection capabilities.
Purpose of the Study:
- To develop a novel mitochondria-targeted fluorescent probe for the simultaneous detection of H₂S, Cys, and GSH.
- To evaluate the probe's performance for in vitro and in vivo applications.
- To explore the potential of the probe as a diagnostic tool for diseases such as tumors.
Main Methods:
- Synthesis and characterization of the near-infrared fluorescent probe HD-Br-D.
- In vitro experiments to assess the probe's selectivity, sensitivity, and response to H₂S, Cys, and GSH.
- Mitochondrial localization studies using fluorescence microscopy.
- In vivo imaging studies in animal models to demonstrate the probe's efficacy.
Main Results:
- The developed probe HD-Br-D exhibits excellent selectivity and sensitivity for simultaneous detection of H₂S, Cys, and GSH.
- HD-Br-D effectively targets mitochondria, allowing for localized intracellular analysis.
- Successful in vitro and in vivo imaging demonstrates the probe's potential for real-time biological monitoring.
- The probe shows promise in distinguishing between normal and diseased tissues in preliminary studies.
Conclusions:
- The mitochondria-targeted fluorescent probe HD-Br-D enables simultaneous detection of H₂S, Cys, and GSH in vitro and in vivo.
- HD-Br-D represents a significant advancement in fluorescent probe technology for biological and medical applications.
- This probe is anticipated to become a valuable tool for the early diagnosis and monitoring of diseases, including tumors.
