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Related Experiment Video

Updated: Sep 11, 2025

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

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Published on: April 4, 2018

A modular and reconfigurable microfluidic device for culturing spheroids under continuous perfusion.

Hiba Aljayyousi, Sarah Sahloul¹, Ajymurat Orozaliev¹

¹Divison of Engineering, New York University Abu Dhabi (NYUAD), Abu Dhabi, United Arab Emirates.

APL Bioengineering

|August 18, 2025

Summary

This study introduces a modular microfluidic device to improve 3D cell spheroid culture. The device enhances spheroid growth and viability, offering a versatile platform for biomedical research and drug testing.

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Last Updated: Sep 11, 2025

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Area of Science:

Biomedical Engineering
Cell Biology
Microfluidics

Background:

3D cell spheroids are vital in vitro models for research.
Diffusion limitations hinder spheroid growth and viability in conventional cultures.

Purpose of the Study:

To develop a versatile, modular microfluidic device for improved spheroid culture.
To enable customizable channel configurations and facile spheroid retrieval.

Main Methods:

A modular microfluidic device with a reconfigurable adhesive layer was designed.
Three channel configurations were tested with Mouse Embryonic Fibroblasts (MEFs), human induced Pluripotent Stem Cells (hiPSCs), and MDA-MB-231 cells.
In situ optical coherence tomography (OCT) was used for noninvasive viability assessment.

Main Results:

The device significantly enhanced spheroid growth (up to 139.9% for MEFs and hiPSCs over 14 days).
Sphericity was maintained for MEF and MDA-MB-231 spheroids; hiPSC spheroids showed budding.
Differential growth rates observed, indicating a need to balance nutrient delivery and autocrine factor retention.

Conclusions:

The modular microfluidic device coupled with OCT is a powerful platform for spheroid culture.
This system advances spheroid culture techniques for applications like drug testing and studying cell interactions.