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Updated: Sep 11, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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RNA-induced Allosteric Coupling Drives Viral Capsid Assembly.

Sean Hamilton1, Tushar Modi1, Petr Šulc1,2,3

  • 1Center for Biological Physics, Arizona State University, Tempe, Arizona 85281, USA.

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Summary
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RNA binding induces allosteric changes in bacteriophage MS2 capsid assembly. This dynamic coupling guides viral capsid formation and offers insights for antiviral and drug delivery systems.

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Area of Science:

  • Biophysics
  • Structural Biology
  • Virology

Background:

  • Single-stranded RNA virus capsid assembly is crucial for viral replication.
  • Understanding these mechanisms can inform antiviral therapies and drug delivery systems.

Purpose of the Study:

  • To investigate RNA-induced allostery in Levivirus bacteriophage MS2 assembly.
  • To elucidate the role of dynamic flexibility and coupling in capsid formation.

Main Methods:

  • Computational analysis using the Dynamic Flexibility Index.
  • Computational analysis using the Dynamic Coupling Index.

Main Results:

  • Asymmetric RNA binding to MS2 coat protein dimers induces conformational changes and reorganizes long-distance communication pathways.
  • A W82R mutation in the FG-loop disrupts communication asymmetry, leading to assembly defects.
  • Dynamic coupling is present in dimers, intermediates, and the final capsid, facilitating signal transduction.

Conclusions:

  • RNA binding triggers allosteric conformational changes essential for MS2 capsid assembly.
  • Dynamic coupling and signal transduction are key mechanisms driving capsid formation.
  • Findings provide a basis for developing novel antiviral strategies and RNA-based delivery vehicles.