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Related Experiment Video

Updated: May 12, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Recent Advances in Functionally Engineered Aptamers: Strategies and Applications.

Mengyue Liu¹, Shicai Xu², Yemin Guo³

¹Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, No. 566 Daxue Xilu, Dezhou, 253023, Shandong, China. mengyueliu@dzu.edu.cn.

Topics in Current Chemistry (Cham)

|August 18, 2025

Summary

Functionally engineered aptamers offer enhanced stability and performance for biosensing applications. This review details their construction, diverse applications, and future potential in overcoming current limitations.

Keywords:

Application Apta-sensing Functionally engineered aptamer Post-SELEX strategy

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Last Updated: May 12, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

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Published on: September 7, 2022

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Aptamers are in vitro selected oligonucleotides with desirable biosensing properties like high affinity and specificity.
Challenges include aptamer flexibility and degradation in biological settings, limiting practical applications.

Purpose of the Study:

To review recent advancements in functionally engineered aptamers.
To summarize construction strategies and sensing applications of engineered aptamers.
To analyze characteristics and multi-field applications of engineered aptamers.

Main Methods:

Review of literature on functionally engineered aptamers.
Analysis of structural modification strategies (e.g., chimera formation, splitting).
Evaluation of aptamer performance in sensing applications.

Main Results:

Engineered aptamers demonstrate improved stability and functionality.
Diverse construction strategies yield aptamers suitable for various sensing platforms.
Engineered aptamers show promise across multiple scientific fields.

Conclusions:

Functional engineering is crucial for overcoming aptamer limitations in biosensing.
Continued research into engineered aptamers holds significant potential for future applications.
Addressing current bottlenecks will further accelerate the development and adoption of engineered aptamers.