Glue-Based Tissue Microarray Construction for Tumor and Disease Research

Tissue microarray technology (TMA) is a high-throughput platform for the simultaneous detection and analysis of multiple tissue samples, facilitating efficient tumor and disease biomarker research. However, conventional TMA construction methods often face limitations such as operational complexity, time-consuming procedures, and variable accuracy. A glue-based TMA construction method was developed to overcome these challenges, offering improved tissue core fixation and enhanced structural stability. Systematic validation included histological evaluation (HE staining), immunohistochemical profiling of target proteins, and fluorescence in situ hybridization (FISH) analysis. Results demonstrated that the glue-based method maintained excellent slice integrity, improved signal-to-noise ratio, and ensured consistent batch-to-batch reproducibility. Although the approach is limited to manual operation, it presents a reliable and cost-effective option for moderate-throughput TMA production. This method is particularly suited to research environments that value flexibility and sample diversity over large-scale automation, expanding the utility of TMA in academic and diagnostic settings.