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Systematically Revealing Quantitative Multi-Target Integrative Effects of Plants With Artificial Intelligence Method.
Jiang Qi-Yu1, Ren Tian-Ai2, Fan Xin-Yu1
1Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Plant Biotechnology Journal
|August 18, 2025
Summary
This study introduces an AI method to analyze how hawthorn
Area of Science:
- Biomedical Science
- Computational Biology
- Pharmacology
Background:
- Plants possess multiple chemical components targeting various disease pathways.
- Understanding the integrated multi-target effects of botanicals on diseases is complex.
- Atherosclerosis involves intricate cellular and molecular interactions within the perivascular adipose tissue (PVAT) microenvironment.
Purpose of the Study:
- To develop a novel artificial intelligence (AI)-based method for assessing the integrated multi-target effects of plants on diseases at a single-cell level.
- To investigate the potential mechanisms and landscape of hawthorn's effects on the atherosclerotic PVAT microenvironment.
Main Methods:
- Utilized a deep auto-encoding neural network to encode single-cell transcriptome data.
- Quantified the integrated multi-target effect of hawthorn on atherosclerosis using a Multi-Target Integrated Score (MTIS).
- Performed single-cell level analyses including MTIS comparisons, pseudotime analysis, cell communication, and immune infiltration.
Main Results:
- Developed and applied a novel AI approach to map the integrated effects of hawthorn on atherosclerotic PVAT at the single-cell level.
- Revealed potential mechanisms underlying hawthorn's influence on the PVAT microenvironment through multi-target analysis.
- Demonstrated the capability of the MTIS to quantitatively assess plant-derived multi-target effects.
Conclusions:
- The developed AI method enables systematic, single-cell level analysis of integrated multi-target effects of botanicals.
- Hawthorn exhibits potential mechanisms influencing the atherosclerotic PVAT microenvironment.
- This novel approach has broad applicability for studying multi-component, multi-target plant-based therapies in various diseases.

