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Updated: Jun 19, 2026

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
Published on: January 11, 2018
Environmental sources of Cryptococcus neoformans species complex in Kampala, Uganda: A preliminary study
Beatrice Achan1, Abel Wembabazi2, Tonny Luggya1
1Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda.
Objectives:
We aimed to determine the environmental sources of C. neoformans and C. gattii species complexes in Uganda.
Methods:
One hundred fifty (n = 150) environmental specimens of chicken droppings, marabou stork bird droppings and eucalyptus tree barks in Uganda were examined phenotypically. The specimens were grown on Sabouraud dextrose agar and the colonies examined by India ink microscopy, urea hydrolysis and formation of blue colonies on chromogenic L-Canavanine glycine blue bromothymol blue agar of C. gattii species complex.
Results:
The prevalence of C. neoformans species complex was 4% (6/150). Of the positive environmental samples from which C. neoformans species complex were isolated; the predominant source was marabou stork droppings; 8% (2/25) followed by eucalyptus tree barks; 3.96% (4/101). However, there was no Cryptococcus gattii species complex; 0% (0/150).
Conclusion:
In Uganda, the environmental sources of C. neoformans species complex are marabou stork droppings and eucalyptus tree barks, however, there seems to be no Cryptococcus gattii species complex.

