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Related Concept Videos

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Related Experiment Video

Updated: Sep 11, 2025

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
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Skin irritation testing using human iPSCs derived 3D skin equivalent model.

Hyewon Shin1, Se-Eun Kim1, C-Yoon Kim1

  • 1College of Veterinary Medicine, Konkuk University, Gwangjin, Seoul, Republic of Korea.

Plos One
|August 18, 2025
PubMed
Summary

Researchers developed a human skin model using stem cells, offering a promising alternative to animal testing for cosmetic and regenerative applications. This 3D skin equivalent model accurately mimics human skin structure and function.

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Dermatology

Background:

  • 3D skin equivalent models (SKEs) are crucial for cosmetic ingredient evaluation and skin regeneration research.
  • Limitations of primary skin cells include donor variability and challenges in genotype-specific studies.
  • Human-induced pluripotent stem cells (hiPSCs) offer a potential solution for creating standardized skin models.

Purpose of the Study:

  • To develop a protocol for differentiating high-purity skin cells (fibroblasts and keratinocytes) from hiPSCs.
  • To construct a functional 3D hiPSC-derived SKE (hiPSC-SKE).
  • To validate the hiPSC-SKE's structural and functional resemblance to native human skin.

Main Methods:

  • Differentiated hiPSCs into high-purity human fibroblasts (hFIBROs) and keratinocytes (hKERAs).
  • Constructed the hiPSC-SKE by layering hKERAs onto a collagen-hFIBROs dermis and inducing keratinization.
  • Performed histological analysis (H&E staining) and assessed functional response to Triton X-100.

Main Results:

  • Successfully differentiated hiPSCs into hFIBROs and hKERAs.
  • The hiPSC-SKE exhibited a layered architecture similar to native human skin with appropriate marker expression.
  • The model showed significant epidermal damage and reduced cell viability upon exposure to Triton X-100, confirming functional responsiveness.

Conclusions:

  • The developed hiPSC-SKE is a viable and reproducible model for skin research.
  • This model offers a promising alternative to animal testing for cosmetic and dermatological applications.
  • The hiPSC-SKE facilitates genotype-specific studies and overcomes limitations of primary cell-based models.