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Related Experiment Video

Updated: May 21, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

Identifying Tipping Points during Healthy Brain Aging through Single-Nucleus Transcriptomic Analysis.

Peiru Wu¹, Xuyu Zhao¹, Zixin Chen¹

¹School of Life Sciences, Shanghai University, Shanghai, 200444, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|August 19, 2025

Summary

This summary is machine-generated.

Healthy brain aging involves shifts in cell populations, with glial cells becoming more unstable. Researchers identified a tipping point for brain aging around 56-60 years, suggesting glia play a key role in this process.

Keywords:

ageing brain cells dynamic network biomarkers nonlinear single‐cell RNA sequencing tipping point

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Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

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Published on: December 22, 2023

Area of Science:

Neuroscience
Systems Biology
Gerontology

Background:

Brain aging impacts cognitive and behavioral functions.
Cellular contributions to age-related brain changes are not fully understood.
Nonlinear aging patterns exist, but cellular dynamics remain unclear.

Purpose of the Study:

To analyze cellular and molecular changes during healthy brain aging.
To identify the tipping point of brain aging at the cellular level.
To explore the role of different cell types in brain aging fluctuations.

Main Methods:

Utilized single-cell transcriptomic data from 45 healthy brain samples (ages 29-94).
Applied nonlinear aging analysis to the cellular level.
Introduced a dynamic network biomarker to identify tipping points.

Main Results:

Neuron proportion decreased slightly but remained stable; glial cells significantly increased and showed instability.
Nonlinear molecular fluctuations peaked at ages 60, 70, and 79.
Identified 56-60 years as a tipping point for healthy brain aging, with glia mediating the process.

Conclusions:

Brain aging exhibits nonlinear dynamics at the cellular level.
Glia are key mediators of the brain's healthy aging tipping point.
This research offers new strategies for early diagnosis and intervention of neurological aging diseases.