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Updated: Sep 10, 2025
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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
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Rapid quantification of immunoglobulin G from plasma using affinity chromatography: partial validation of an
Markus Mozgovicz1,2, Alois Jungbauer1,2, Nico Lingg1,2
1Department of Biotechnology and Food Science, Institute of Bioprocess Science and Engineering, BOKU University, Vienna, Austria.
Preparative Biochemistry & Biotechnology
|August 19, 2025
Summary
This study validates a rapid affinity chromatography method for quantifying immunoglobulin G (IgG). The validated method offers a faster, more reliable alternative to traditional nephelometry for IgG analysis in human plasma.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Chromatography
Background:
- Affinity chromatography offers advantages over nephelometry for immunoglobulin G (IgG) quantification.
- Existing methods require optimization for speed and efficiency.
Purpose of the Study:
- To validate the analytical application of POROS CaptureSelect FcXP affinity resin for IgG quantification.
- To establish a fast, reliable, and precise method for IgG measurement.
Main Methods:
- Utilized POROS CaptureSelect FcXP affinity resin in a bind-elute mode.
- Optimized residence time to achieve short cycle times (2.5-5 minutes).
- Validated linearity, precision, and recovery using human plasma samples.
Main Results:
- Achieved a cycle time as low as 2.5 minutes.
- Demonstrated linearity from 4 µg to 260 µg IgG.
- Exhibited excellent precision (%RSD 1-5%) and high recovery (average 99%).
- Enabled direct quantification of IgG from human plasma without dilution.
Conclusions:
- The validated affinity chromatography method is efficient and reliable for IgG quantification.
- This method presents significant advantages over nephelometry, including reduced analysis time.
- The method is suitable for high-throughput workflows and direct analysis of human plasma.
