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Updated: Sep 10, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Two novel mitoviruses isolated from the filamentous fungus Gnomoniopsis sanguisorbae
Zhenmei Song1,2, Juan Yang1, Lina Zeng2
1School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, 561113, China.
Abstract:
In this study, we isolated two novel mitoviruses co-infecting the fungus Gnomoniopsis sanguisorbae, which were designated as "Gnomoniopsis sanguisorbae mitovirus L" (GsMV-L) and "Gnomoniopsis sanguisorbae mitovirus M" (GsMV-M). The complete genome lengths of GsMV-L and GsMV-M were determined to be 2,785 and 2,529 nucleotides, respectively, with a GC content of 44% and 35%, respectively. Both viruses possess a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp) containing conserved catalytic motifs. BLASTp analysis revealed that the RdRps of GsMV-L and GsMV-M exhibited the highest amino acid sequence similarity to those of sunflower phomopsis-associated mitovirus 1 and Phomopsis viticola mitovirus 962_1, with 50.97% and 41.97% identity, respectively. Phylogenetic analysis based on RdRp sequences indicated that GsMV-L clustered with members of the genus Triamitovirus, while GsMV-M clustered with members of the genus Unuamitovirus, both of which are taxonomically classified within the family Mitoviridae.
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