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Related Concept Videos

Western Blotting01:15

Western Blotting

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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Video

Updated: May 5, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Next-generation leukemia diagnostics: Integrating LC-MS/MS proteomics with liquid biopsy platforms.

Vivek Singh1

  • 1Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, 226003, India.

The Journal of Liquid Biopsy
|August 20, 2025
PubMed
Summary

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) liquid biopsy offers a less invasive way to detect leukemia early. Proteomic analysis of blood biomarkers shows promise for diagnosing T-cell acute lymphoblastic leukemia and acute myeloid leukemia.

Keywords:
Biomarker discoveryCirculating protein biomarkersLC-MS/MSLeukemia diagnosisLiquid biopsyMulti-omicsProteomicsT-ALL

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Area of Science:

  • Oncology
  • Biochemistry
  • Proteomics

Background:

  • Leukemia diagnosis traditionally relies on invasive bone marrow biopsies.
  • Liquid biopsy, analyzing blood biomarkers, offers a less invasive alternative for early detection.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a sensitive technology for blood protein profiling.

Purpose of the Study:

  • To review the clinical potential of LC-MS/MS-based liquid biopsy for leukemia diagnosis.
  • To focus on applications in T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML).
  • To discuss challenges and future directions for clinical translation.

Main Methods:

  • Review of recent proteomic studies utilizing LC-MS/MS for leukemia biomarker discovery.
  • Analysis of protein signatures identified in blood samples.
  • Discussion of technological and clinical implementation challenges.

Main Results:

  • Distinct protein signatures (e.g., XRRA1, CPNE4, S100A8) identified in T-ALL patients show diagnostic potential.
  • LC-MS/MS enables high-throughput and sensitive detection of leukemia-associated proteins.
  • Emerging proteomic data supports liquid biopsy for leukemia detection.

Conclusions:

  • LC-MS/MS liquid biopsy is a promising tool for earlier, less invasive leukemia diagnostics.
  • Clinical implementation requires validation in larger cohorts, standardization, and regulatory approval.
  • Integration with multi-omics platforms may enhance diagnostic precision.