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Related Experiment Video

Updated: May 2, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Morphological changes after corneal stromal lenticule excimer laser shaping.

Xiaoxuan Wang¹, Xiaopeng Liu¹, Jie Hou²

¹Department of Ophthalmology, Binzhou Medical University, Binzhou, Shandong 256603, P.R. China.

Experimental and Therapeutic Medicine

|August 20, 2025

Summary

This summary is machine-generated.

Excimer laser cutting of corneal lenticules in vitro demonstrated that different cutting ratios significantly alter central and 1.5 mm thickness. A 1:2 ratio produced a concave lenticule, indicating precise shape control is achievable.

Keywords:

OCT concave lenticule corneal stromal lenticule excimer laser cutting keratoconus parallel lenticule

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Area of Science:

Ophthalmology
Laser Surgery
Biomedical Engineering

Background:

Corneal lenticule creation is key for refractive surgery.
Excimer laser technology offers precision in tissue ablation.
Understanding lenticule geometry is crucial for surgical outcomes.

Purpose of the Study:

To assess the feasibility of creating parallel/concave lenticules.
To investigate the impact of different excimer laser cutting ratios on lenticule shape.
To analyze corneal stromal lenticule thickness changes in vitro.

Main Methods:

Corneal lenticules were prepared in vitro on a convex matrix.
Excimer laser cutting was performed at 1:1, 1:1.5, and 1:2 ratios.
Optical coherence tomography measured lenticule thickness before and after cutting.

Main Results:

Significant thickness changes occurred centrally and at 1.5 mm with varying ratios.
Thickness changes at 2 and 2.5 mm from the center were not statistically significant.
A 1:1 ratio yielded a convex lenticule, 1:1.5 a near-parallel convex shape, and 1:2 a concave lenticule.

Conclusions:

Excimer laser cutting proportions influence lenticule thickness profiles.
The 1:2 cutting ratio successfully produced a concave lenticule shape.
This study demonstrates controlled lenticule shaping for potential refractive applications.