Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

5.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.7K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

5.9K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

S-phase targeted treatment triggers caspase-dependent lytic immunogenic cell death with pyroptotic features in cancers.

Cell death and differentiation·2026
Same author

Large distant deletion disrupts CDKN2A enhancer and predisposes to melanoma.

medRxiv : the preprint server for health sciences·2026
Same author

Personalized targeting of BCL2 family proteins overcomes acquired resistance to BRAF-MEK inhibitors in preclinical melanoma.

Nature communications·2026
Same author

Meeting Report From the 2025 Cure Ocular Melanoma (CURE OM) Global Science Meeting, Amsterdam, the Netherlands, October 2025.

Pigment cell & melanoma research·2026
Same author

Translational case series comparing next-generation sequencing profiles of primary breast cancer and brain metastases.

Cancer treatment and research communications·2026
Same author

Topical resiquimod elicits systemic protection and improves anti-PD1 therapy in melanoma via priming of CD8+ T cells.

Cancer immunology research·2026

Related Experiment Video

Updated: Sep 8, 2025

A Melanoma Patient-Derived Xenograft Model
07:07

A Melanoma Patient-Derived Xenograft Model

Published on: May 20, 2019

12.5K

Modelling Acral Melanoma in Admixed Brazilians Uncovers Genomic Drivers and Targetable Pathways.

Annie Cristhine Moraes Sousa-Squiavinato1, Sara Santos Bernardes2, Flávia C Aguiar1

  • 1Division of Basic and Experimental Research, Brazilian National Cancer Institute, Rua Andre Cavalcanti 37, Rio de Janeiro, 20231-050, Brazil.

Medrxiv : the Preprint Server for Health Sciences
|August 20, 2025
PubMed
Summary

Acral melanoma (AM) exhibits distinct genomic features, including copy number alterations called hailstorms, and shows sensitivity to targeted therapies. This study provides new models and identifies novel vulnerabilities for this aggressive skin cancer.

More Related Videos

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.5K
Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

13.8K

Related Experiment Videos

Last Updated: Sep 8, 2025

A Melanoma Patient-Derived Xenograft Model
07:07

A Melanoma Patient-Derived Xenograft Model

Published on: May 20, 2019

12.5K
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.5K
Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

13.8K

Area of Science:

  • Oncology
  • Genomics
  • Cancer Biology

Background:

  • Acral melanoma (AM) is an aggressive skin cancer subtype with poor outcomes and limited treatment options.
  • AM disproportionately affects non-European and admixed populations, including those in Latin America.

Purpose of the Study:

  • To perform comprehensive genomic and functional profiling of acral melanoma in a diverse Brazilian cohort.
  • To establish patient-derived xenograft (AM-PDX) models for exploring AM biology and therapeutic vulnerabilities.

Main Methods:

  • Whole-exome and transcriptome sequencing of AM tumors.
  • Establishment and characterization of acral melanoma patient-derived xenograft (AM-PDX) models.
  • Pharmacological and CRISPR/Cas9 knockout screening for functional dependencies.

Main Results:

  • AM tumors show low mutation burden but frequent copy number alterations, including 'hailstorms' affecting oncogenes (e.g., CCND1, CDK4) and DNA damage response genes (e.g., ATM).
  • AM-PDX models recapitulate key histopathological and genomic features of primary tumors.
  • Functional screenings identified sensitivities to MAPK, CDK4/6, MDM2, and WEE1 pathway inhibitors, with RMC-7977 effective against RAS/KIT-mutant AM.
  • CRISPR screens revealed novel AM-specific vulnerabilities, including CRKL and SF3B4.

Conclusions:

  • Acral melanoma possesses a distinct genomic profile compared to other melanoma subtypes.
  • The developed AM-PDX models offer a valuable resource for studying AM, particularly in populations of Latin American ancestry.
  • Targeting specific pathways (MAPK, CDK4/6, MDM2, WEE1) and novel vulnerabilities (CRKL, SF3B4) presents promising therapeutic strategies for acral melanoma.