Related Experiment Video
Updated: Sep 10, 2025

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate
Kaiyu Gao1, Hui Zhang1, Yidong Liu1
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, P. R. China.
None:
Glutarate is a platform chemical with diversified applications. It is also an endogenous metabolite involved in various physiological processes. Deficiency in glutaryl-CoA dehydrogenase (GcdH) for glutarate catabolism induces the inherited metabolic disorder glutaric aciduria. In this study, a genetically encoded glutarate fluorescent biosensor Glusor is constructed and optimized based on transcriptional regulator CsiR and circularly permuted yellow fluorescent protein. Glusor can quantify glutarate in human body fluids and bacteria fermentation broth with good accuracy and precision, supporting the convenient diagnosis of glutaric aciduria and glutarate production monitoring. Then, the glutarate transport is characterized independent of radioactive substrate by using Glusor expressed in Escherichia coli. The functions of transporters KgtP and YnfM in the uptake and efflux of glutarate in E. coli are identified. Glusor is also used to reveal the catabolism of glutarate in bacteria and HEK293FT cells. The role of glutarate hydroxylase in glutarate catabolism of E. coli is identified through Glusor-supported in situ glutarate detection. Spatially resolved in vivo analysis of glutarate in HEK293FT cells is realized and GcdH inhibition and hypoxia-induced glutarate accumulation are elucidated by using Glusor. Overall, Glusor is a versatile tool for the detection of glutarate both in vitro and in vivo.
Related Concept Videos
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Secondary Active Transport
Membrane Proteins
The Significance of Membrane Transport
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...

