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Natalia A Plotnitskaya1,2, Anastasiia V Krivoruchko1,2, Irina B Ivshina1,2

  • 1Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Perm, Russia.

Microbiology Resource Announcements
|August 20, 2025
PubMed

Keywords:
Rhodococcus rhodochrousbetulinbiotransformation

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
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