SOX15 Transcriptionally Decreases the Level of MMP2 and Inhibits Vasculogenic Mimicry to Slow Down the Progression of Ovarian Cancer
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View abstract on PubMed
Summary
This summary is machine-generated.Sex-determining region Y-box 15 (SOX15) suppresses ovarian cancer progression by inhibiting vascular mimicry. SOX15 directly represses matrix metalloproteinase-2 (MMP2) expression, offering a new therapeutic target.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Research
Background
- Vascular mimicry (VM) promotes tumor growth and invasion in ovarian cancer.
- Sex-determining region Y-box 15 (SOX15) is known to suppress tumor growth, but its role in ovarian cancer is unclear.
Purpose Of The Study
- To investigate the function of SOX15 in ovarian cancer cell proliferation, invasion, and vascular mimicry.
- To elucidate the underlying molecular mechanisms of SOX15 action in ovarian cancer.
Main Methods
- Utilized SKOV-3 and ES2 ovarian cancer cell lines and xenograft mouse models.
- Assessed cell proliferation, migration, and invasion.
- Measured VE-cadherin, VEGFA, Ki67, and MMP2 expression.
- Performed dual-luciferase reporter and ChIP-PCR assays to determine SOX15's mechanism of action.
Main Results
- SOX15 overexpression inhibited proliferation, migration, and invasion in ovarian cancer cells.
- SOX15 reduced VM formation by downregulating VE-cadherin and VEGFA.
- SOX15 knockdown increased tumor growth and VM formation in vivo.
- SOX15 directly repressed MMP2 promoter activity and expression.
Conclusions
- SOX15 acts as a tumor suppressor in ovarian cancer by transcriptionally repressing MMP2.
- Inhibiting VM formation through the SOX15/MMP2 axis presents a potential therapeutic strategy for ovarian cancer.
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