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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Sep 10, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

Are Solid Particles Ready for Prime-Time Proteomics?

Eduardo S Kitano^1,2, Yana Demyanenko^1,2, Shabaz Mohammed^1,3,4

¹Rosalind Franklin Institute, Harwell Campus, OX11 0QX Didcot, United Kingdom.

Analytical Chemistry

|August 20, 2025

Summary

This summary is machine-generated.

Nonporous C-18 particle columns offer efficient peptide separations for high-speed proteomics. These columns perform comparably to traditional materials in nano-UHPLC and nano-HPLC systems.

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Last Updated: Sep 10, 2025

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Published on: July 12, 2014

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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

Area of Science:

Analytical Chemistry
Biochemistry
Chromatography

Background:

High-throughput proteomics demands efficient separation techniques.
Conventional chromatography relies on fully porous (FPP) and superficially porous particles (SPP).
Nonporous particle (NPP) materials offer potential advantages in speed and efficiency.

Purpose of the Study:

To evaluate the performance of nonporous C-18 stationary phases in high-speed proteomics workflows.
To assess the suitability of NPP columns for nano-UHPLC and nano-HPLC systems.
To compare NPP column performance against FPP and SPP materials.

Main Methods:

Fabrication of analytical columns using 1.0-1.5 μm nonporous C-18 particle materials (SOLAD and ODS-IIIE).
Compatibility testing with nano-UHPLC and nano-HPLC pressure regimes using 150 μm i.d. fused silica capillaries.
Evaluation of peptide separation efficiency using varying column lengths (15-25 cm) and optimized HPLC methods (Evosep One Whisper Zoom).
Performance comparison via DIA LC-MS/MS analysis of human cell lysate digests.

Main Results:

Both ODS-IIIE and SOLAD NPP materials supported efficient peptide separations in nano-UHPLC systems.
Shorter NPP columns achieved competitive separation performance (FWHM < 2-3 s) comparable to FPP and SPP columns.
DIA LC-MS/MS analysis showed NPP columns performed comparably or slightly better than FPP and SPP materials.

Conclusions:

Nonporous particle (NPP) based columns are a viable alternative to FPP and SPP materials for proteomics.
NPP columns are particularly well-suited for high-throughput and high-sensitivity proteomics applications.
These findings support the adoption of NPP columns in advanced proteomics workflows.