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Systemic lactate kinetics during graded exercise in man.

W C Stanley, E W Gertz, J A Wisneski

    The American Journal of Physiology
    |December 1, 1985
    PubMed
    Summary
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    This study examined how oxygen consumption (VO2) relates to lactate appearance (Ra) and disappearance (Rd) during graded exercise. Lactate production exceeded clearance at higher exercise intensities, indicating an imbalance during intense physical activity.

    Area of Science:

    • Exercise Physiology
    • Metabolic Biochemistry

    Background:

    • Systemic lactate kinetics are crucial for understanding exercise metabolism.
    • The relationship between oxygen consumption and lactate turnover during graded exercise requires further elucidation.

    Purpose of the Study:

    • To investigate the dynamic relationships between oxygen consumption (VO2) and the rates of systemic lactate appearance (Ra) and disappearance (Rd) during continuous graded exercise.
    • To quantify lactate production and clearance across a range of exercise intensities in healthy males.

    Main Methods:

    • Utilized a primed continuous infusion of L-(+)-[1-14C]lactate tracer in six healthy males.
    • Subjects performed 6-minute bouts of continuous graded exercise (300, 600, 900, 1,200 kg·m·min⁻¹).
    • Calculated non-steady-state Ra and Rd using arterial blood samples collected at rest and during exercise.

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    Main Results:

    • At rest, lactate appearance and disappearance rates were similar (14.4 ± 1.8 vs. 15.1 ± 2.2 μmol·kg⁻¹·min⁻¹).
    • Near steady-state conditions were observed at lower exercise intensities (300 and 600 kg·m·min⁻¹), with Ra and Rd increasing significantly.
    • At higher intensities (900 and 1,200 kg·m·min⁻¹), lactate appearance significantly exceeded disappearance (e.g., 309.4 ± 20.8 vs. 169.7 ± 10.6 μmol·kg⁻¹·min⁻¹ at 1,200 kg·m·min⁻¹).

    Conclusions:

    • Lactate kinetics shift during graded exercise, with production surpassing clearance at higher intensities.
    • This imbalance suggests a critical threshold where lactate accumulation begins during strenuous physical exertion.
    • The findings provide insights into the metabolic regulation of lactate during exercise and its relationship with oxygen consumption.