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Ultrastructural Visualization of the Nuclear Envelope in HeLa Cells.
Sourabh Sengupta1, Zhaojie Zhang2, Daniel L Levy3
1Department of Molecular Biology, University of Wyoming, Laramie, WY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2025
Summary
Transmission electron microscopy (TEM) visualizes cellular structures. This study details a TEM method for observing the nuclear envelope in HeLa cells, aiding research into its ultrastructure under various conditions.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Transmission electron microscopy (TEM) has visualized biological materials since the 1940s.
- TEM offers detailed observation of cellular organelles, intracellular structures, and viruses.
- Insights into cell nucleus structure and function have been gained using TEM.
Purpose of the Study:
- To present a method for visualizing the nuclear envelope in cultured HeLa cells using TEM.
- To provide a comprehensive protocol from cell culturing to visualization.
- To enable the study of nuclear envelope ultrastructure alterations due to cell culture conditions, genetic modifications, and stress.
Main Methods:
- Cell culturing of HeLa cells.
- Fixation, embedding, sectioning, and staining protocols.
- Visualization using transmission electron microscopy.
Main Results:
- A detailed method for TEM visualization of the nuclear envelope is established.
- The protocol allows for high-resolution imaging of nuclear envelope ultrastructure.
- The method is applicable to various experimental conditions.
Conclusions:
- The presented TEM method is effective for detailed nuclear envelope visualization in HeLa cells.
- This technique facilitates research on how external factors influence nuclear envelope ultrastructure.
- The study provides a valuable tool for cell biology research.
