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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
Published on: April 3, 2015
Micropost Arrays to Model ECM Fiber Obstacles During Cell Migration in Confinement
Aditya Katiyar1, Richard B Dickinson2, Tanmay P Lele3,4,5
1Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA. adityakatiyar@tamu.edu.
None:
Micropost arrays are a valuable platform for studying cell migration in controlled microenvironments. These arrays enable researchers to mimic how migrating cells deform their nuclei in reaction to physical constraints presented by extracellular matrix (ECM) fibers during processes such as wound healing and cancer metastasis. By tuning micropost patterns and sizes, researchers can not only explore the impact of confining spaces created by such patterns on cell migration, signaling, and nuclear deformation but also simultaneously quantify cell-generated forces and forces of nuclear deformation. This paper outlines methods to design and fabricate ECM fiber mimicking micropost arrays for use in fundamental studies of confining cell migration.
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