Related Experiment Video
Updated: Sep 10, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
Published on: September 6, 2024
Use of Nucleoporin-Conjugated Beads to Study the Nuclear Pore Complex Assembly on the Nuclear Membrane
Şükriye Bilir1,2, Yasushi Hiraoka1, Tokuko Haraguchi3
1Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Abstract:
Reassembly of the nuclear pore complex (NPC) at the end of mitosis is a key event for establishing a functional nuclear envelope. However, the role of each NPC component (Nup) in NPC reassembly remained unclear. Here, we describe a method using artificial beads conjugated with an anti-GFP antibody. In this method, such beads are introduced into living cells expressing the Nup of interest fused with GFP to tether the specific Nup around the beads. By examining the Nup-conjugated beads in mitotic telophase cells, it is possible to investigate the role of the specific Nup of interest in NPC formation on the nuclear membrane.
Related Concept Videos
Nuclear Protein Sorting
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Regulation of Nuclear Protein Sorting

