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Published on: March 8, 2012
Detection of human papillomavirus in male genital samples
Saúl A Villasmil1, Jorgen A Marcano2,3,4, José A Briceño5,6
1Clinical Research and Molecular Biology Laboratory (LICBIOMOL), Libertador Avenue, Siclar building, 2nd floor, office 22, Caracas, Venezuela. saul.villasmil@ucv.ve.
Purpose:
This study focuses on the detection of human papillomavirus (HPV) in genital samples from heterosexual men, with or without HPV-like lesions, using molecular biology techniques for diagnosis and genotyping of the virus.
Method:
A retrospective observational study was conducted with 294 male patients who visited a laboratory for molecular testing, with a presumptive clinical-epidemiological diagnosis of HPV infection, without prior HPV disease or vaccination. Samples were analyzed using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques.
Results:
Detection of HPV-infected men was 50.8%, with significant variability in incidence depending on the anatomical site of sampling. Fourteen high-risk HPV types were identified, along with the presence of multiple subtypes in several patients.
Conclusion:
This study provides valuable insights into HPV incidence in men, the most prevalent genotypes, and their correlation with lesion presence. These findings may aid in developing prevention, vaccination, and control strategies for HPV in men.
