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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Related Experiment Video

Updated: Sep 10, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Metabolite Functions Revealed by Mass Spectrometry-Based Target Engagement Proteomics Approaches.

Nisha A Owens1, Tayah B Sommer1, Brenna G Ing1

  • 1Department of Biology, University of Prince Edward Island, Charlottetown C1A 4P3, Canada.

Journal of Proteome Research
|August 20, 2025
PubMed
Summary

Endogenous metabolites have critical roles beyond energy and building blocks, influencing cell functions through protein interactions. New research highlights these secondary functions, paving the way for understanding complex biological networks and disease targets.

Keywords:
mass spectrometrymetabolite-protein interactionsmetabolomeproteometarget engagement techniques

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Related Experiment Videos

Last Updated: Sep 10, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Systems Biology

Background:

  • Endogenous metabolites play crucial roles in cellular energetics and biosynthesis.
  • Emerging evidence reveals novel bioactive functions of metabolites in cancer, immunity, and host-pathogen interactions.
  • Understanding metabolite-protein interactions is key to characterizing the metabolome-proteome intersection.

Purpose of the Study:

  • To summarize recently identified secondary functions of metabolites.
  • To highlight mass spectrometry-based approaches for small molecule target engagement.
  • To propose advancements in metabolome-proteome interaction network analysis.

Main Methods:

  • Mass spectrometry-based approaches for small molecule target engagement.
  • Review of recently uncovered secondary metabolite functions.
  • Analysis of metabolite-protein interactions.

Main Results:

  • Metabolites exert functional roles through protein interactions, including allosteric and transcriptional regulation.
  • Secondary functions of metabolites are being uncovered using advanced mass spectrometry techniques.
  • These interactions are critical for understanding cellular processes and disease mechanisms.

Conclusions:

  • Defining metabolite-protein interactions reveals downstream effects and advances metabolome-proteome network understanding.
  • Further development of target engagement approaches will enhance metabolite function discovery.
  • This research may identify new therapeutic targets for diseases like cancer.