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Decoding the proteomic landscape of platelet extracellular vesicles: Implications for coagulation in burn patients
1Department of burn surgery, burn medical institute of Inner Mongolia, the third affiliated hospital of Inner Mongolia medical university, Baogang hospital, Baotou, China.
Background:
To analyze the effects of platelet extracellular vesicles (PLT-EVs) on coagulation function in severe burns patients using in vitro coagulation assays, and to explore the proteins involved in coagulation reaction in PLT-EVs by proteomics technology, laying the foundation for the diagnosis and treatment of coagulation dysfunction in patients with severe burns.
Methods:
Six healthy volunteers and six patients with severe burns were selected. The coagulation function was compared by thromboelastography and conventional coagulation tests, and the differentially expressed proteins of PLT-EVs in the control group and the burns group were analyzed by liquid chromatography-mass spectrometry. The differentially expressed proteins were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG); the top 10 proteins related to coagulation were screened.
Results:
The in vitro coagulation results revealed that the severe burns patients were in a hypocoagulable state following shock, as evidenced by hyperfibrinolysis and decreased platelet function and quantity; PLT-EVs were found to promote coagulation in vitro. Quantitative analysis of the 853 proteins found in PLT-EVs indicated that 191 proteins were up-regulated and 77 proteins were down-regulated. The GO and KEGG analysis of the differentially expressed proteins showed their involvement in biological processes such as coagulation function, inflammatory response, immune response, and body repair and regulation.
Conclusions:
PLT-EVs play a role in reducing coagulation dysfunction after severe burns by regulating the quantity of coagulation-related proteins.
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