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Improved nitrogen removal from real mariculture effluent by halotolerant bacterial consortium SND223: Preparation,
Xin Su1, Shihan Wang1, Xiaoqian Cheng1
1Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, Dalian Ocean University, Dalian 116023, China; College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China.
Abstract:
This study developed a halotolerant composite bio-agent (SND223) containing Acinetobacter B2, B3, and Zobellella sp. MAD-44 (2:2:3) for saline aquaculture wastewater treatment. Optimised at carbon-to-nitrogen (C/N) 10, 150 r/m, and 30 °C, SND223 achieved complete ammonia (100 %) and high nitrate (97.1 %) removal within 24 h, with 62.1 % gaseous nitrogen conversion and 20.1 % intracellular nitrogen assimilation. Immobilized PVA-SA/DIA-Z@SND223 beads boosted the efficiency of moving bed biofilm reactor (MBBR) in saline aquaculture wastewater treatment, maintaining low ammonia levels (<0.2 mg/L) in effluent, elevating nitrate removal by 32.1 % (cyclic mode) and 17.1 % (once-through mode) with a short hydraulic retention time (2 h). Scanning electron microscopy (SEM) confirmed the porous architecture of beads supported microbial proliferation, while sequencing revealed successful colonisation of denitrifying genera Acinetobacter and Zobellella in MBBR biofilms, accompanied by upregulation of key functional genes (amoA, hao, nirS, nosZ). The findings reveal the bioaugmentation mechanisms of SND223 for saline wastewater treatment.
