Intracytoplasmic sperm injection-mediated strategy for the production of transgenic poultry as a bioreactor

A number of methods are used to produce recombinant proteins, and animal bioreactors have emerged as transgenic systems. Animal bioreactors have the potential to reduce production costs and improve efficiency, thereby providing recombinant proteins that are important for therapeutic applications. Various species, such as goats, cattle, and rabbits, have been genetically modified to serve as bioreactors. Since poultry hens produce more than 300 eggs per year, their eggs may also be used as an efficient platform for the production of protein pharmaceuticals. In addition to traditional viral infection of the blastoderm, the recent development of genome-editing technologies for cultured primordial germ cells has enabled the establishment of transgenic chicken lines via germline chimera production systems. We established a new genome-editing technology combined with the intracytoplasmic sperm injection (ICSI) method that has the potential to produce transgenic quail very efficiently in one generation. The avian ICSI technique and recent advances in genome editing are discussed herein.