Microwave Asisted Synthesis of Methylene Bisthiazolo Arylvinyl Pyrazoles as Potential Biological Agents

(2E,2'E)-1,1'-(5,5'-(5,5'-methylenebis(2-methoxy-5,1-phenylene))bis(3-(4-fluorophenyl)-6-phenyl-3,3a,5,6-tetrahydro-2H-pyrazolo[3,4-d]thiazole-5,2-diyl))bis(3-phenylprop-2-en-1-one) were synthesized from (5Z,5'Z)-2,2'-(5,5'-methylenebis(2-methoxy-5,1-phenylene))bis(5-(4-fluorobenzylidene)-3-phenylthiazolidin-4-one)through cycloaddition reaction with Cinnamic acid hydrazide and evaluated its anti bacterial, fungal and nematicidal activity against. Among the tested compounds 5b and 5d containing chloro and Nitro are the most effective against Dietylenchus myceliophagus, Caonorbitis elegans with LD 50 between 160 and 190 ppm, 190 and 210 ppm respectively, . Compounds 5b, 5e showed good zone of inhibition against B. subtiles compounds 5c and 5d were active against S.aureus compounds 5a, 5c &5e were active against C. Violaceum and compounds 5b, 5a and 5d were the most active against K. aerogenes, P. aeruginosa and B. sphaericus. Compounds 5b and 5d showed good inhibition towards C. albicans at the concentration of  3.12 μg/mL which is less than Amphotericin B standard.