Micellar Liquid Chromatographic Method for Determination of Moxifloxacin and its Impurities
Alma Salkić1, Mira Zečević2, Biljana Otašević3
1ZADA Pharmaceuticals d.o.o. Bistarac Donji bb, 75300 Lukavac, Bosnia and Herzegovina Faculty of Pharmacy, University of Tuzla, Studentska 6, 75000 Tuzla, Bosnia and Herzegovina. alma.avdic.salkic@zada.ba.
Abstract:
A selective eco-friendly micellar HPLC method for investigation of moxifloxacin and related substances in presence of its degradation products, was developed. Central Composite Design was used to optimize the experimental conditions. The proposed method is based on isocratic elution on a C18 column, using 92.5% (v/v) biodegradable aqueous mobile phase containing 0.01 M sodium dihydrogenphosphate,0.15 M sodium dodecyl sulfate (SDS) and 0.5% triethylamine (v/v) with pH value set at 3.5 and 7.5% of propanol (v/v) as eco-friendly organic solvent. The flow rate and injection volume were 0.6 ml/min and 5 µl, respectively. Experiments were carried out at temperature 60 °C and detection was performed at 295 nm. The optimized method was validated.The method was found to be suitable for quantification of moxifloxacin and its related substances in moxifloxacin drug substance. The Green analytical procedure index (GAPI) demonstrated the superiority of the developed method.
More Related Videos
08:57Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
Published on: October 5, 2017
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
Related Concept Videos
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Differential Staining Technique
Special Staining Techniques
