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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Updated: May 7, 2026

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A Rapid Assessment Approach for Skin Stratum-Targeted Drug Delivery Systems Using Mass Spectrometry Imaging and

Ravit Yakobi Arancibia1, Einav Bentov-Arava1, Anna Morshin1

  • 1The Institute for Drug Research, the School of Pharmacy Faculty of Medicine The Center for Nanoscience and Nanotechnology The Hebrew University of Jerusalem Jerusalem 9112001 Israel.

Small Science
|August 21, 2025
PubMed
Summary

A new mass spectrometry imaging method rapidly visualizes drug distribution in skin layers. This automated tool enhances accuracy and efficiency for evaluating drug delivery systems, showing transethosomes offer deeper dermal penetration.

Keywords:
dermal deliverydesorption electrospray ionization mass spectrometry imagingethosomesmicroemulsionskin layersterbinafinetransethosomes

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Area of Science:

  • Pharmacology and Pharmaceutical Sciences
  • Analytical Chemistry
  • Biomedical Imaging

Background:

  • Evaluating drug distribution in skin layers is crucial for dermal delivery systems.
  • Current methods for analyzing drug permeation are often time-consuming and lack precision.
  • Novel imaging techniques are needed for efficient and accurate assessment of active pharmaceutical ingredient (API) localization.

Purpose of the Study:

  • To present a novel mass spectrometry imaging (MSI)-based concept for rapid visualization and evaluation of API distribution in skin.
  • To develop and validate an automated computational tool for efficient MSI data processing and skin layer segmentation.
  • To compare the dermal delivery performance of three distinct nanoscale drug delivery systems (DDSs) using the developed MSI method.

Main Methods:

  • Integration of desorption electrospray ionization MSI with a newly developed automated computational tool.
  • Efficient processing of MSI data to isolate skin tissue signals and segment precise skin layers.
  • Design and characterization of three nanoscale DDSs (ethosomes, transethosomes, microemulsion) for the antifungal terbinafine.
  • Evaluation of API permeation in human and porcine skin using both manual and automated MSI workflows.

Main Results:

  • The integrated MSI approach demonstrated superior accuracy in skin distribution analysis, reducing processing time to under 10 minutes per specimen.
  • Significant improvements in signal-tissue overlay and efficiency were observed compared to manual methods.
  • Comparative analysis revealed distinct differences in drug permeation depth and localization among the DDSs.
  • Transethosomes exhibited the highest potential for deeper dermal delivery of terbinafine.

Conclusions:

  • The developed MSI-based concept and automated tool provide a powerful, rapid, and accurate method for evaluating DDS performance.
  • This approach enables detailed kinetic studies and offers insights into drug permeation dynamics within skin strata.
  • The findings highlight the potential of transethosomes for enhanced dermal drug delivery, targeting deeper skin layers.