Annotated draft genome data of Bacillus safensis GMA1 isolated from hot springs in Mexico reveals its xylanolytic...

Bacillus species are excellent industrial organisms due to their high growth rate and capacity to secrete enzymes into the culture medium. The B. pumilus group is one of the most widely studied groups and a potential source of industrial enzymes, and includes B. safensis, among others. B. safensis GMA1 was isolated from hot springs at Los Azufres, Michoacán, Mexico. Microscopical observations revealed that it is a Gram-positive, spore-forming, rod-shaped bacterium. B. safensis GMA1 has shown protease, lipase, cyanide dihydratase, and xylanase activities. Whole-genome sequencing was performed on the NovaSeq 6000 Illumina platform using a 250-bp paired-end protocol. The genome of B. safensis GMA1 consists of 36 contigs, with a GC content of 41.58%. A total of 3.7 Mbp (3,736,651 bp) of genomic size was generated. Genome annotation resulted in the prediction of 3842 genes, comprising 3732 coding sequences (CDS), 74 tRNA genes, and 31 rRNA genes. This work reports sequencing, assembly, and annotation of the B. safensis GMA1 genome, highlighting the potential of this strain as a source of xylanolytic enzymes. The required enzymes for degrading xylan were identified through genome analysis. Enzymes as 1,4-beta-xylosidase (EC 3.2.1.37), endo-1,4-beta-xylanase (EC 3.2.1.8), acetylxylan esterase (EC 3.1.1.72), arabinoxylan arabinofuranohydrolase (EC 3.2.1.55), and glucuronoarabinoxylan endo-1,4-beta-xylanase (EC 3.2.1.136) were found.