Silver Nanoparticles and Houttuynin Sodium-Based Fibrous Poly(L-lactide-co-glycolide)/Gelatin-Based Dressings for...

Bacterial infections present debilitating challenges for skin repair, particularly in managing complex microbial colonization and persistent inflammation that delay healing. To address this clinical gap, a dual-functional electrospun dressing (PG@AgH) was developed by incorporating silver nanoparticles (Ag NPs) and houttuynin sodium (H) into poly(L-lactide-co-glycolide)/gelatin (PG) fibers. Compared to conventional dual-agent systems, PG@AgH achieves spatiotemporally controlled release. Ag NPs provide rapid antibacterial action against multidrug-resistant pathogens (e.g., S. aureus, E. coli), while H sustains anti-inflammatory modulation through macrophage polarization regulation. In vitro studies confirmed cytocompatibility and broad-spectrum antimicrobial efficacy against bacterial (S. aureus, E. coli) and fungal (C. albicans) strains. Moreover, the PG@AgH dressings promoted rapid wound closure, enhanced collagen deposition, and reduced bacterial load at the wound site in S. aureus-infected full-thickness excisional defect model in rats. Taken together, this multifunctional approach overcomes the limitations of single-mechanism dressings, offering a clinically translatable strategy for infectious wound management.