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Integration of Multi-Rotor and Multi-Conjugation: Ultrahigh Absorption Coefficient Dye Enables NIR-II Fluorescence

Guining Cao1, Jiacheng Tang1, Meijing Wang1

  • 1School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, 510060, China.

Small (Weinheim an Der Bergstrasse, Germany)
|August 21, 2025
PubMed

Insights

This study introduces TPE-CyA nanoparticles for near-infrared II photothermal immunotherapy. These nanoparticles effectively suppress tumor growth and metastasis by inducing immunogenic cell death and activating systemic immunity.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Research

Background:

  • Cancer cells accumulate mutations, leading to immune escape.
  • Photothermal immunotherapy triggers immunogenic cell death (ICD) and releases neoantigens.
  • Near-infrared II (NIR-II) region offers advantages for deep tissue imaging and therapy.

Purpose of the Study:

  • To design novel cyanine molecules for NIR-II photothermal immunotherapy.
  • To evaluate the efficacy of TPE-CyA nanoparticles in cancer treatment and imaging.
  • To investigate the immunomodulatory effects of NIR-II photothermal immunotherapy.

Main Methods:

  • Designed NIR-II cyanine molecules based on the "multi-rotor & multi-conjugation" concept.
  • Synthesized and characterized TPE-CyA nanoparticles (TPE-CyA NPs).
  • Evaluated TPE-CyA NPs for NIR-II fluorescence imaging, angiography, and photothermal immunotherapy in vivo.

Main Results:

  • TPE-CyA exhibited high molar extinction coefficient and photothermal conversion efficiency.
  • TPE-CyA NPs enabled high-resolution NIR-II imaging and angiography.
  • Imaging-guided TPE-CyA NPs suppressed bilateral tumor growth and liver metastasis, inducing ICD and systemic immunity.

Conclusions:

  • TPE-CyA nanoparticles represent a promising strategy for NIR-II photothermal immunotherapy.
  • This approach activates anti-tumor immunity, preventing metastasis and recurrence.
  • Highlights the potential of NIR-II photothermal therapy for cancer treatment and immune activation.
Keywords:
NIR‐II cyanine moleculesNIR‐II photothermal materialsfluorescence imagephotothermal immunotherapytumor metastasis

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