Colorimetric sensor array based on a bimetallic PtPd nanozyme for simultaneous discrimination of multiple...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Antioxidant species are crucial for redox balance and disease markers, but traditional methods lack specificity for complex biological systems.
• Colorimetric sensor arrays offer a powerful solution for simultaneous detection and differentiation of multiple, similar analytes.
• Developing advanced sensing platforms is essential for accurate biomedical diagnostics and health monitoring.

Purpose of the Study:

• To develop a triple-channel colorimetric sensor array for simultaneous qualitative and quantitative analysis of multiple antioxidants.
• To utilize a bimetallic PtPd nanozyme with peroxidase-mimicking activity for enhanced sensing capabilities.
• To validate the sensor array's performance in complex biological samples and mixtures.

Main Methods:

• A triple-channel colorimetric sensor array was designed using a bimetallic PtPd nanozyme.
• 3,3',5,5'-tetramethylbenzidine (TMB) served as the chromogenic substrate.
• Three distinct pH conditions were employed as independent sensing channels to generate differential responses for antioxidant identification.

Main Results:

• The sensor array successfully discriminated and quantified five antioxidants: ascorbic acid, citric acid, cysteine, tannic acid, and uric acid.
• The system demonstrated excellent selectivity, sensitivity, and reproducibility in complex sample matrices.
• Accurate identification of target antioxidants was achieved in human serum and various multicomponent mixtures.

Conclusions:

• The PtPd nanozyme-based colorimetric sensor array offers a versatile and promising platform for antioxidant analysis.
• The developed system exhibits strong discriminative capability and applicability for real-world samples.
• This technology holds significant potential for biomedical diagnostics, disease biomarker screening, and health monitoring.