The effect of C-reactive protein on the PHA-induced proliferation of human peripheral blood lymphocytes

The effect of CRP purified from ascites fluid originated from cancer patients on human peripheral blood lymphocytes was investigated. CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. CRP was able either to inhibit or to enhance PHA-induced lymphocyte proliferation in vitro. The effects of added CRP depended on the time of its addition, dose of CRP and lymphocyte donor.