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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Sep 10, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Imaging-based multimodal profiling of single cells with STAMP

Kellie Wise^1,2, Anna Pascual-Reguant^3,4

¹Adelaide Centre for Epigenetics, School of Biomedicine, The University of Adelaide, Adelaide, South Australia, Australia. kellie.wise@adelaide.edu.au.

Nature Reviews. Genetics

|August 21, 2025

Summary

No abstract available in PubMed .

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