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Related Experiment Video

Updated: Jul 11, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Autonomous cryoprotectant loading of the oocyte using microfluidic transistors.

Li Zhan^1,2, Hunter Hinnen^1,3, Kaustav A Gopinathan^1,2

¹Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Boston, MA, USA.

Device

|August 22, 2025

Summary

This study introduces a low-cost microfluidic device automating oocyte cryopreservation for in vitro fertilization (IVF). The device simplifies cryoprotective agent loading, aiming to improve IVF accessibility and affordability.

Keywords:

Microfluidic transistor autonomous device cryopreservation cryoprotectant loading oocyte

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Last Updated: Jul 11, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

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Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

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Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

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Published on: November 11, 2022

Area of Science:

Biotechnology
Reproductive Biology
Microfluidics

Background:

Oocyte cryopreservation is vital for in vitro fertilization (IVF) but relies on manual methods.
Manual cryoprotectant loading in IVF is prone to variations, high costs, and limited accessibility.

Purpose of the Study:

To develop a low-cost, user-friendly microfluidic device for automating oocyte cryoprotective agent (CPA) loading.
To create a device that delivers cryopreservation-ready oocytes for assisted reproductive technologies.

Main Methods:

Utilized microfluidic transistors to engineer fluidic timers and logic gates.
Employed time-dependent pressure signals to control microfluidic valves for regulating oocyte exposure to solutions.
Demonstrated autonomous oocyte trapping, CPA loading, dehydration, and extraction using constant pressure.

Main Results:

Successfully automated the complex, multistep process of oocyte CPA loading.
Achieved autonomous mouse oocyte trapping, equilibrium solution loading, vitrification solution dehydration, and extraction.
The microfluidic device delivered cryopreservation-ready oocytes.

Conclusions:

The developed microfluidic device offers a viable solution for automating oocyte cryopreservation.
This technology represents a significant advancement towards autonomous "IVF-on-a-chip" systems.
The innovation aims to enhance the accessibility and affordability of IVF treatments globally.