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Immobilized Black Radish Peroxidase on ZnO-Alginate Beads: A Multifunctional Biocatalyst for Sustainable Food
1Faculty of Science, Department of Chemistry Ataturk University Erzurum Turkiye.
Abstract:
This study presents a novel bioactive food packaging material developed by immobilizing black radish peroxidase (Br-POD) calcium alginate beads loaded with zinc oxide nanoparticles (ZnO/CA). The immobilization process achieved an efficiency of 90.6% and increased enzyme-specific activity by 36%. The ZnO/CA-Br-POD composite exhibited enhanced thermal stability (optimal temperature shift from 40°C to 60°C) and pH tolerance (from pH 6 to 5), along with strong antimicrobial activity against Staphylococcus aureus and Escherichia coli. Antioxidant performance, evaluated via DPPH and ABTS radical scavenging assays, showed up to 93.4% activity. Application on Agaricus bisporus mushrooms reduced weight loss by 49.5% during storage, demonstrating significant preservation capacity. These findings underscore the potential of ZnO/CA-Br-POD composites as multifunctional, eco-friendly materials for active food packaging applications.
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